pbluescript sk ii (+) vector plasmid (Agilent technologies)
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pbluescript sk ii (+) vector plasmid
Pbluescript Sk Ii (+) Vector Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+sk+(+)+vector/pm39082859-55-10-16
Average 90 stars, based on 1 article reviews
Pbluescript Sk Ii (+) Vector Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+sk+(+)+vector/pm39082859-55-10-16
Average 90 stars, based on 1 article reviews
pbluescript sk ii (+) vector plasmid - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta Article Snippet: .. Both were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the Article Title: The Transposon Galileo Generates Natural Chromosomal Inversions in Drosophila by Ectopic Recombination Article Snippet: .. DNA fragments of interest from positive phages were subcloned into Article Title: Identification of sugar response complex in the metallothionein OsMT2b gene promoter for enhancement of foreign protein production in transgenic rice. Article Snippet: Key message A 146-bp sugar response complex MTSRC is identified in the promoter of rice metallothionein OsMT2b gene conferring high-level expression of luciferase reporter gene and bioactive recombinant haFGF in transgenic rice.. Abstract A rice subfamily type 2 plant metallothionein (pMT) gene, OsMT2b, encoding a reactive oxygen species (ROS) scavenger protein, has been previously shown to exhibit the most abundant gene expression in young rice seedling.. Expression of OsMT2b was found to be regulated negatively by ethylene and hydrogen peroxide in rice stem node under flooding stress, but little is known about its response to sugar depletion. Article Title: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri Article Snippet: The lengths of PCR products were determined by comparison with DNA size markers (100 bp DNA marker, Fermentas, St. Leon-Rot, Germany and 1 kb DNA ladder, Invitrogen, Carlsbad, CA). .. Products of PCR amplification were cloned into the Article Title: Leishmania major Methionine Sulfoxide Reductase A Is Required for Resistance to Oxidative Stress and Efficient Replication in Macrophages Article Snippet: .. An overlap PCR was then performed using these PCR products as template, and the resultant product cloned into the HindIII/NotI sites of the Article Title: Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth. Article Snippet: Plasmids All PCR steps were performed using Tks Gflex DNA Polymerase (TaKaRa). .. Human PTPRZ-long (6948 bp) was cloned by real-time PCR using total RNA from LN-229 cells with the primers 5′-GGAATTCGATATCAAATGCGAATCCTAAAGCGTTT-3′ (forward) and 5′- ACGGTATCGATAAGCTTTAAACTAAAGACTCTAAG-3′ (reverse) and subcloned into the EcoRV site of the Sequencing:Article Title: Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta Article Snippet: .. Both were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the Synthesized:Article Title: Identification of sugar response complex in the metallothionein OsMT2b gene promoter for enhancement of foreign protein production in transgenic rice. Article Snippet: Key message A 146-bp sugar response complex MTSRC is identified in the promoter of rice metallothionein OsMT2b gene conferring high-level expression of luciferase reporter gene and bioactive recombinant haFGF in transgenic rice.. Abstract A rice subfamily type 2 plant metallothionein (pMT) gene, OsMT2b, encoding a reactive oxygen species (ROS) scavenger protein, has been previously shown to exhibit the most abundant gene expression in young rice seedling.. Expression of OsMT2b was found to be regulated negatively by ethylene and hydrogen peroxide in rice stem node under flooding stress, but little is known about its response to sugar depletion. Polymerase Chain Reaction:Article Title: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri Article Snippet: The lengths of PCR products were determined by comparison with DNA size markers (100 bp DNA marker, Fermentas, St. Leon-Rot, Germany and 1 kb DNA ladder, Invitrogen, Carlsbad, CA). .. Products of PCR amplification were cloned into the Article Title: Leishmania major Methionine Sulfoxide Reductase A Is Required for Resistance to Oxidative Stress and Efficient Replication in Macrophages Article Snippet: .. An overlap PCR was then performed using these PCR products as template, and the resultant product cloned into the HindIII/NotI sites of the Amplification:Article Title: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri Article Snippet: The lengths of PCR products were determined by comparison with DNA size markers (100 bp DNA marker, Fermentas, St. Leon-Rot, Germany and 1 kb DNA ladder, Invitrogen, Carlsbad, CA). .. Products of PCR amplification were cloned into the Clone Assay:Article Title: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri Article Snippet: The lengths of PCR products were determined by comparison with DNA size markers (100 bp DNA marker, Fermentas, St. Leon-Rot, Germany and 1 kb DNA ladder, Invitrogen, Carlsbad, CA). .. Products of PCR amplification were cloned into the Article Title: Leishmania major Methionine Sulfoxide Reductase A Is Required for Resistance to Oxidative Stress and Efficient Replication in Macrophages Article Snippet: .. An overlap PCR was then performed using these PCR products as template, and the resultant product cloned into the HindIII/NotI sites of the Article Title: Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth. Article Snippet: Plasmids All PCR steps were performed using Tks Gflex DNA Polymerase (TaKaRa). .. Human PTPRZ-long (6948 bp) was cloned by real-time PCR using total RNA from LN-229 cells with the primers 5′-GGAATTCGATATCAAATGCGAATCCTAAAGCGTTT-3′ (forward) and 5′- ACGGTATCGATAAGCTTTAAACTAAAGACTCTAAG-3′ (reverse) and subcloned into the EcoRV site of the Real-time Polymerase Chain Reaction:Article Title: Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth. Article Snippet: Plasmids All PCR steps were performed using Tks Gflex DNA Polymerase (TaKaRa). .. Human PTPRZ-long (6948 bp) was cloned by real-time PCR using total RNA from LN-229 cells with the primers 5′-GGAATTCGATATCAAATGCGAATCCTAAAGCGTTT-3′ (forward) and 5′- ACGGTATCGATAAGCTTTAAACTAAAGACTCTAAG-3′ (reverse) and subcloned into the EcoRV site of the Cloning:Article Title: Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth. Article Snippet: Plasmids All PCR steps were performed using Tks Gflex DNA Polymerase (TaKaRa). .. Human PTPRZ-long (6948 bp) was cloned by real-time PCR using total RNA from LN-229 cells with the primers 5′-GGAATTCGATATCAAATGCGAATCCTAAAGCGTTT-3′ (forward) and 5′- ACGGTATCGATAAGCTTTAAACTAAAGACTCTAAG-3′ (reverse) and subcloned into the EcoRV site of the Marker:Article Title: A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences Article Snippet: .. Chemicals, enzymes and strains High-fidelity Taq DNA polymerase pyrobest , T4 DNA ligase, DL2000 DNA marker, restriction endonucleases, IPTG and X-Gal were purchased from Takara Co., Ltd (Dalian, People's Republic of China). Article Title: A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences Article Snippet: .. High-fidelity Taq DNA polymerase pyrobest , T4 DNA ligase, DL2000 DNA marker, restriction endonucleases, IPTG and X-Gal were purchased from Takara Co., Ltd (Dalian, People's Republic of China). |